An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The BAX-binding protein MOAP1 associates with LC3 and promotes closure of the phagophore.

Chang HC, Tao RN, Tan CT, Wu YJ, Bay BH, Yu VC

MOAP1 (modulator of apoptosis 1) is a BAX-binding protein tightly regulated by the ubiquitin-proteasome system. Apoptotic stimuli stabilize MOAP1 protein and facilitate its interaction with BAX to promote apoptosis. Here we show that in contrast to being resistant to apoptotic stimuli, MOAP1-deficient cells are hypersensitive to cell death mediated by starvation rendered by EBSS treatment. MOAP1-deficient cells exhibited impairment in ... [more]

Autophagy Mar. 30, 2021; ();1-15 [Pubmed: 33783314]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MAP1LC3B MTCH2	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Zellner S (2021)	High	-	BioGRID	3306988

Curated By

BioGRID

Interaction Summary

MAP1LC3B

Gene Ontology Biological Process

Gene Ontology Molecular Function

GABA receptor binding [IBA]microtubule binding [IBA]protein binding [IPI]

Gene Ontology Cellular Component

MTCH2