BAIT

STP22

AGS1, VPL15, VPS23, ubiquitin-binding ESCRT-I subunit protein STP22, L000003975, S000029031, L000000064, YCL008C
Component of the ESCRT-I complex; ESCRT-I is involved in ubiquitin-dependent sorting of proteins into the endosome; prevents polyubiquitination of the arrestin-related protein Rim8p, thereby directing its monoubiquitination by Rsp5p; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype;
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

IST2

YBR086C
Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process
Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

ESCRT-III and ER-PM contacts maintain lipid homeostasis.

Jorgensen JR, Tei R, Baskin JM, Michel AH, Kornmann B, Emr SD

Eukaryotic cells are compartmentalized into organelles by intracellular membranes. While the organelles are distinct, many of them make intimate contact with one another. These contacts were first observed in the 1950s, but only recently have the functions of these contact sites begun to be understood. In yeast, the endoplasmic reticulum (ER) makes extensive intermembrane contacts with the plasma membrane (PM), ... [more]

Mol Biol Cell Dec. 01, 2019; 31(12);1302-1313 [Pubmed: 32267208]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • Figure 1
  • delta-tether has six deletions (scs2 scs22 tcb1 tcb2 tcb3 ist2)
  • genetic complex
  • vps23 deletion shows synthetic growth defect with delta-tether strain

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
STP22 IST2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1523BioGRID
2085587

Curated By

  • BioGRID