Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

COMMD4 functions with the histone H2A-H2B dimer for the timely repair of DNA double-strand breaks.

Suraweera A, Gandhi NS, Beard S, Burgess JT, Croft LV, Bolderson E, Naqi A, Ashton NW, Adams MN, Savage KI, Zhang SD, O'Byrne KJ, Richard DJ

Genomic stability is critical for normal cellular function and its deregulation is a universal hallmark of cancer. Here we outline a previously undescribed role of COMMD4 in maintaining genomic stability, by regulation of chromatin remodelling at sites of DNA double-strand breaks. At break-sites, COMMD4 binds to and protects histone H2B from monoubiquitination by RNF20/RNF40. DNA damage-induced phosphorylation of the H2A-H2B ... [more]

Commun Biol Dec. 19, 2020; 4(1);484 [Pubmed: 33875784]

Throughput

  • Low Throughput

Curated By

  • BioGRID