Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Expression of mRNA and protein-protein interaction of the antiviral endoribonuclease RNase L in mouse spleen.

Gupta A, Rath PC

The interferon-inducible, 2',5'-oligoadenylate (2-5A)-dependent endoribonuclease, RNase L is a unique antiviral RNA-degrading enzyme involved in RNA-metabolism, translational regulation, stress-response besides its anticancer/tumor-suppressor and antibacterial functions. RNase L represents complex cellular RNA-regulations in mammalian cells but diverse functions of RNase L are not completely explained by its 2-5A-regulated endoribonuclease activity. We hypothesized that RNase L has housekeeping function(s) through interaction with ... [more]

Int J Biol Macromol Aug. 01, 2014; 69();307-18 [Pubmed: 24780566]

Throughput

  • Low Throughput

Curated By

  • BioGRID