BAIT
PRKACA
PKCD, Pkaca
protein kinase, cAMP dependent, catalytic, alpha
GO Process (20)
GO Function (10)
GO Component (16)
Gene Ontology Biological Process
- cellular response to glucose stimulus [ISO]
- cellular response to parathyroid hormone stimulus [IMP]
- mesoderm formation [IGI]
- negative regulation of meiotic cell cycle [ISO]
- negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning [IGI]
- neural tube closure [IGI]
- peptidyl-serine phosphorylation [IDA, ISO]
- peptidyl-threonine phosphorylation [IDA]
- positive regulation of cell cycle arrest [IDA]
- positive regulation of protein export from nucleus [IMP]
- protein autophosphorylation [IDA]
- protein phosphorylation [IDA, ISO]
- regulation of cellular respiration [ISO]
- regulation of osteoblast differentiation [ISO]
- regulation of proteasomal protein catabolic process [ISO]
- regulation of protein processing [IGI]
- regulation of synaptic transmission [IMP]
- regulation of synaptic transmission, glutamatergic [ISO]
- regulation of tight junction assembly [ISO]
- sperm capacitation [IDA]
Gene Ontology Molecular Function- Rab GTPase binding [ISO]
- cAMP-dependent protein kinase activity [IDA, ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase A regulatory subunit binding [ISO]
- protein kinase activity [IDA]
- protein kinase binding [ISO]
- protein serine/threonine kinase activity [IDA]
- protein serine/threonine/tyrosine kinase activity [ISO]
- ubiquitin protein ligase binding [ISO]
- Rab GTPase binding [ISO]
- cAMP-dependent protein kinase activity [IDA, ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase A regulatory subunit binding [ISO]
- protein kinase activity [IDA]
- protein kinase binding [ISO]
- protein serine/threonine kinase activity [IDA]
- protein serine/threonine/tyrosine kinase activity [ISO]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
- AMP-activated protein kinase complex [ISO]
- Golgi apparatus [ISO]
- cAMP-dependent protein kinase complex [ISO]
- centrosome [ISO]
- ciliary base [IDA]
- cytoplasm [IDA, ISO]
- extracellular vesicular exosome [ISO]
- membrane [ISO]
- mitochondrion [IDA]
- neuromuscular junction [IDA]
- neuron projection [ISO]
- nucleus [IDA, ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [IDA]
- protein complex [ISO]
- sperm midpiece [ISO]
Mus musculus
PREY
AKAP5
3526401B18Rik, AKAP 150, AKAP-5, AKAP150, BB098886, Gm258, P150
A kinase (PRKA) anchor protein 5
GO Process (11)
GO Function (12)
GO Component (13)
Gene Ontology Biological Process
- cellular protein complex disassembly [ISO]
- negative regulation of adenylate cyclase activity [ISO]
- negative regulation of ion transport [ISO]
- negative regulation of potassium ion transport [ISO]
- positive regulation of NFAT protein import into nucleus [ISO]
- positive regulation of adenylate cyclase activity [ISO]
- positive regulation of cAMP biosynthetic process [ISO]
- positive regulation of calcium ion transport into cytosol [ISO]
- positive regulation of dendrite morphogenesis [ISO]
- positive regulation of protein kinase A signaling [ISO]
- positive regulation of protein phosphorylation [ISO]
Gene Ontology Molecular Function- G-protein coupled receptor binding [ISO]
- GABA receptor binding [ISO]
- actin binding [ISO]
- adenylate cyclase binding [ISO]
- cadherin binding [ISO]
- kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein domain specific binding [ISO]
- protein kinase A regulatory subunit binding [ISO]
- protein kinase binding [ISO]
- protein phosphatase 2B binding [ISO]
- G-protein coupled receptor binding [ISO]
- GABA receptor binding [ISO]
- actin binding [ISO]
- adenylate cyclase binding [ISO]
- cadherin binding [ISO]
- kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein domain specific binding [ISO]
- protein kinase A regulatory subunit binding [ISO]
- protein kinase binding [ISO]
- protein phosphatase 2B binding [ISO]
Gene Ontology Cellular Component
- asymmetric synapse [ISO]
- basolateral plasma membrane [ISO]
- cytoplasm [ISO]
- dendrite membrane [ISO]
- dendritic shaft [ISO]
- dendritic spine [ISO]
- dendritic spine membrane [ISO]
- filopodium membrane [ISO]
- neuronal cell body [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [ISO]
- postsynaptic density [ISO]
- protein complex [ISO]
Mus musculus
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Probing cellular protein complexes using single-molecule pull-down.
Proteins perform most cellular functions in macromolecular complexes. The same protein often participates in different complexes to exhibit diverse functionality. Current ensemble approaches of identifying cellular protein interactions cannot reveal physiological permutations of these interactions. Here we describe a single-molecule pull-down (SiMPull) assay that combines the principles of a conventional pull-down assay with single-molecule fluorescence microscopy and enables direct visualization ... [more]
Nature May. 26, 2011; 473(7348);484-8 [Pubmed: 21614075]
Throughput
- Low Throughput
Additional Notes
- SiMPull
Curated By
- BioGRID