An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

SARS-CoV-2-host proteome interactions for antiviral drug discovery.

Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, Poehner I, Oehman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S, Vartiainen MK, Pietiaeinen V, Poso A, Varjosalo M

Treatment options for COVID-19, caused by SARS-CoV-2, remain limited. Understanding viral pathogenesis at the molecular level is critical to develop effective therapy. Some recent studies have explored SARS-CoV-2-host interactomes and provided great resources for understanding viral replication. However, host proteins that functionally associate with SARS-CoV-2 are localized in the corresponding subnetwork within the comprehensive human interactome. Therefore, constructing a downstream ... [more]

Mol Syst Biol Dec. 01, 2020; 17(11);e10396 [Pubmed: 34709727]

Quantitative Score

0.9 [Saint Score]

Throughput

High Throughput

Additional Notes

BioID
High confidence interactions are assigned based on their statistical filtering score (BFDR =< 0.01) and further refined using the CRAPome contaminant repository.
The associated score is the original published SAINT score determined by the authors using Significance Analysis of INTeractome (SAINT) express version 3.6.0.

Curated By

BioGRID

Interaction Summary

TFRC

Gene Ontology Biological Process

Gene Ontology Molecular Function

double-stranded RNA binding [IDA]identical protein binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]transferrin receptor activity [NAS]

Gene Ontology Cellular Component

ARL1

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTP binding [IDA]GTPase activity [IDA]enzyme activator activity [IDA]protein domain specific binding [IPI]