BAIT

NSP13

ORF1ab, ORF1ab-nsp13, Hel, SARS-CoV2 nsp13, SARS-CoV-2 nsp13, Helicase, R1AB_SARS2, PRO_0000449630, GU280_gp01_nsp13
Helicase; Non-structural protein 13
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

RDX

DFNB24
radixin
GO Process (2)
GO Function (2)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

SARS-CoV-2-host proteome interactions for antiviral drug discovery.

Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, Poehner I, Oehman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S, Vartiainen MK, Pietiaeinen V, Poso A, Varjosalo M

Treatment options for COVID-19, caused by SARS-CoV-2, remain limited. Understanding viral pathogenesis at the molecular level is critical to develop effective therapy. Some recent studies have explored SARS-CoV-2-host interactomes and provided great resources for understanding viral replication. However, host proteins that functionally associate with SARS-CoV-2 are localized in the corresponding subnetwork within the comprehensive human interactome. Therefore, constructing a downstream ... [more]

Mol Syst Biol Dec. 01, 2020; 17(11);e10396 [Pubmed: 34709727]

Quantitative Score

  • 0.99 [Saint Score]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • High confidence interactions are assigned based on their statistical filtering score (BFDR =< 0.01) and further refined using the CRAPome contaminant repository.
  • The associated score is the original published SAINT score determined by the authors using Significance Analysis of INTeractome (SAINT) express version 3.6.0.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NSP13 RDX
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3480297
NSP13 RDX
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3802177
NSP13 RDX
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.912BioGRID
2652434
NSP13 RDX
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.912BioGRID
2875383
NSP13 RDX
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High-BioGRID
3804182
NSP13 RDX
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
3501273

Curated By

  • BioGRID