An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Faciogenital Dysplasia 5 supports cancer stem cell traits in basal-like breast cancer by enhancing EGFR stability.

Li K, Zhang TT, Zhao CX, Wang F, Cui B, Yang ZN, Lv XX, Yeerjiang Z, Yuan YF, Yu JM, Wang ZH, Zhang XW, Yu JJ, Liu SS, Shang S, Huang B, Hua F, Hu ZW

Most basal-like breast cancers (BLBCs) are triple-negative breast cancers (TNBCs), which have the worst prognosis and distant metastasis-free survival among breast cancer subtypes. Now, no targeted therapies are available for patients with BLBC due to the lack of reliable and effective molecular targets. Here, we performed the BLBC tissue microarray-based immunohistochemical analysis and showed that Faciogenital Dysplasia 5 (FGD5) abundance ... [more]

Sci Transl Med Dec. 24, 2020; 13(586); [Pubmed: 33762435]

Quantitative Score

6589.78 [Confidence Score]

Throughput

High Throughput

Additional Notes

Affinity capture-MS was carried out to identify high-confidence protein interactions.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PLEC FGD5	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Fasci D (2018)	High	-	BioGRID	2784125

Curated By

BioGRID

Interaction Summary

FGD5

Gene Ontology Biological Process

Gene Ontology Molecular Function

guanyl-nucleotide exchange factor activity [ISS]protein binding [IPI]small GTPase binding [ISS]

Gene Ontology Cellular Component

PLEC