An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Faciogenital Dysplasia 5 supports cancer stem cell traits in basal-like breast cancer by enhancing EGFR stability.

Li K, Zhang TT, Zhao CX, Wang F, Cui B, Yang ZN, Lv XX, Yeerjiang Z, Yuan YF, Yu JM, Wang ZH, Zhang XW, Yu JJ, Liu SS, Shang S, Huang B, Hua F, Hu ZW

Most basal-like breast cancers (BLBCs) are triple-negative breast cancers (TNBCs), which have the worst prognosis and distant metastasis-free survival among breast cancer subtypes. Now, no targeted therapies are available for patients with BLBC due to the lack of reliable and effective molecular targets. Here, we performed the BLBC tissue microarray-based immunohistochemical analysis and showed that Faciogenital Dysplasia 5 (FGD5) abundance ... [more]

Sci Transl Med Dec. 24, 2020; 13(586); [Pubmed: 33762435]

Quantitative Score

1383.82 [Confidence Score]

Throughput

High Throughput

Additional Notes

Affinity capture-MS was carried out to identify high-confidence protein interactions.

Curated By

BioGRID

Interaction Summary

FGD5

Gene Ontology Biological Process

Gene Ontology Molecular Function

guanyl-nucleotide exchange factor activity [ISS]protein binding [IPI]small GTPase binding [ISS]

Gene Ontology Cellular Component

ALDOA

Gene Ontology Biological Process

Gene Ontology Molecular Function

actin binding [TAS]cytoskeletal protein binding [IDA]fructose binding [IDA]fructose-bisphosphate aldolase activity [IDA]identical protein binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]tubulin binding [TAS]