BAIT

AGO1

EIF2C, EIF2C1, GERP95, Q99, RP4-789D17.1
argonaute RISC catalytic component 1
Homo sapiens
PREY

IGF2BP1

CRD-BP, CRDBP, IMP-1, IMP1, VICKZ1, ZBP1
insulin-like growth factor 2 mRNA binding protein 1
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.

Hoeck J, Weinmann L, Ender C, Ruedel S, Kremmer E, Raabe M, Urlaub H, Meister G

Members of the Argonaute (Ago) protein family associate with small RNAs and have important roles in RNA silencing. Here, we analysed Ago1- and Ago2-containing protein complexes in human cells. Separation of Ago-associated messenger ribonucleoproteins (mRNPs) showed that Ago1 and Ago2 reside in three complexes with distinct Dicer and RNA-induced silencing complex activities. A comprehensive proteomic analysis of Ago-containing mRNPs identified ... [more]

EMBO Rep. Nov. 01, 2007; 8(11);1052-60 [Pubmed: 17932509]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
IGF2BP1 AGO1
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Low-BioGRID
2818026

Curated By

  • BioGRID