BAIT

TOPBP1

TopBP1-A, TopBP1-B, Xmus101, XtopBP, cut5, mus101, top2bp1
topoisomerase (DNA) II binding protein 1
GO Process (0)
GO Function (0)
GO Component (0)
Xenopus laevis
PREY

ATR

XATR, frp1, mec1, sckl, sckl1
ATR serine/threonine kinase
GO Process (0)
GO Function (0)
GO Component (0)
Xenopus laevis

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Ataxia-telangiectasia mutated (ATM)-dependent activation of ATR occurs through phosphorylation of TopBP1 by ATM.

Yoo HY, Kumagai A, Shevchenko A, Shevchenko A, Dunphy WG

ATM (ataxia-telangiectasia mutated) is necessary for activation of Chk1 by ATR (ATM and Rad3-related) in response to double-stranded DNA breaks (DSBs) but not to DNA replication stress. TopBP1 has been identified as a direct activator of ATR. We show that ATM regulates Xenopus TopBP1 by phosphorylating Ser-1131 and thereby strongly enhancing association of TopBP1 with ATR. Xenopus egg extracts containing ... [more]

J Biol Chem Jun. 15, 2007; 282(24);17501-6 [Pubmed: 17446169]

Throughput

  • Low Throughput

Curated By

  • BioGRID