ARRB2
Gene Ontology Biological Process
- G-protein coupled receptor internalization [IDA, IMP]
- Notch signaling pathway [TAS]
- blood coagulation [TAS]
- cell chemotaxis [IMP]
- desensitization of G-protein coupled receptor protein signaling pathway by arrestin [IMP]
- negative regulation of NF-kappaB transcription factor activity [IDA]
- negative regulation of natural killer cell mediated cytotoxicity [IMP]
- negative regulation of protein ubiquitination [IDA]
- platelet activation [TAS]
- positive regulation of ERK1 and ERK2 cascade [IDA, IMP]
- positive regulation of protein ubiquitination [IGI]
- positive regulation of receptor internalization [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
- protein ubiquitination [IMP]
- receptor internalization [IDA]
- regulation of androgen receptor signaling pathway [IDA]
- transcription from RNA polymerase II promoter [IDA]
- transforming growth factor beta receptor signaling pathway [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ITCH
Gene Ontology Biological Process
- Notch signaling pathway [TAS]
- inflammatory response [NAS]
- innate immune response [TAS]
- negative regulation of JNK cascade [ISS]
- negative regulation of NF-kappaB transcription factor activity [ISS]
- negative regulation of apoptotic process [IMP]
- negative regulation of defense response to virus [IMP]
- negative regulation of type I interferon production [TAS]
- nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway [TAS]
- nucleotide-binding oligomerization domain containing signaling pathway [TAS]
- protein K29-linked ubiquitination [IDA]
- protein K48-linked ubiquitination [IDA]
- protein K63-linked ubiquitination [IDA]
- protein ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IBA]
- regulation of cell growth [NAS]
- regulation of protein deubiquitination [ISS]
- ubiquitin-dependent protein catabolic process [IDA, NAS]
- viral entry into host cell [TAS]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Nedd4 mediates agonist-dependent ubiquitination, lysosomal targeting, and degradation of the beta2-adrenergic receptor.
Agonist-stimulated beta(2)-adrenergic receptor (beta(2)AR) ubiquitination is a major factor that governs both lysosomal trafficking and degradation of internalized receptors, but the identity of the E3 ubiquitin ligase regulating this process was unknown. Among the various catalytically inactive E3 ubiquitin ligase mutants that we tested, a dominant negative Nedd4 specifically inhibited isoproterenol-induced ubiquitination and degradation of the beta(2)AR in HEK-293 cells. ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ITCH ARRB2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ITCH ARRB2 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID