BAIT
UBP3
BLM3, mRNA-binding ubiquitin-specific protease UBP3, L000002417, S000086717, YER151C
Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress; role in ribophagy
GO Process (4)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
MRC1
YCL060C, chromatin-modulating protein MRC1, YCL061C
S-phase checkpoint protein required for DNA replication; couples DNA helicase and DNA polymerase; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; with Hog1p defines a novel S-phase checkpoint that permits eukaryotic cells to prevent conflicts between DNA replication and transcription; protects uncapped telomeres; degradation via Dia2p help cells resume cell cycle
GO Process (11)
GO Function (0)
GO Component (4)
Gene Ontology Biological Process
- DNA repair [IGI, IMP]
- DNA replication [IMP]
- DNA replication checkpoint [IGI, IMP, IPI]
- chromatin silencing at silent mating-type cassette [IGI, IMP]
- chromatin silencing at telomere [IGI, IMP]
- intra-S DNA damage checkpoint [IMP]
- maintenance of DNA repeat elements [IMP]
- mitotic sister chromatid cohesion [IGI, IMP]
- regulation of nuclear cell cycle DNA replication [IMP]
- replication fork protection [IGI, IMP, IPI]
- telomere maintenance [IMP]
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Functional organization of the S. cerevisiae phosphorylation network.
Reversible protein phosphorylation is a signaling mechanism involved in all cellular processes. To create a systems view of the signaling apparatus in budding yeast, we generated an epistatic miniarray profile (E-MAP) comprised of 100,000 pairwise, quantitative genetic interactions, including virtually all protein and small-molecule kinases and phosphatases as well as key cellular regulators. Quantitative genetic interaction mapping reveals factors working ... [more]
Cell Mar. 06, 2009; 136(5);952-63 [Pubmed: 19269370]
Quantitative Score
- -2.698242 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) analysis was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score > 2.0 for positive interactions (suppression) and S score < -2.5 for negative interactions (synthetic sick/lethality).
Curated By
- BioGRID