An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The BioPlex Network of Human Protein Interactions: Additional Unpublished AP-MS Results (Pre-Publication)

Huttlin EL, Pontano-Vaites L, Navarrete-Perea J, Bruckner RJ, Gebreab F, Gygi MP, Thornock A, Fu S, Maenpaa E, Golbazi A, Stricker K, Guha Thakurta S, Zhang T, Rad R, Paulo JA, Harper JW, Gygi SP

As part of an ongoing effort led by Steve Gygi, Wade Harper, and Ed Huttlin in the Department of Cell Biology at Harvard Medical School, we are systematically profiling the interactions among human proteins using affinity purification mass spectrometry. In this effort, HA-tagged bait proteins obtained from the human ORFeome collection (version 8.1; Marc Vidal) are expressed individually in human ... [more]

Status: Pre-Publication Dataset

Quantitative Score

0.937522662 [compPASS Score]

Throughput

High Throughput

Additional Notes

BioPlex HCT (unpublished interaction)
BioPlex HCT HCT116 cells CompPASS score = 0.937522661539104, threshold = 0.362. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.362 threshold represents the top 2% of scores in HCT116.

Curated By

BioGRID

Interaction Summary

SIK1

Gene Ontology Biological Process

Gene Ontology Molecular Function

14-3-3 protein binding [IDA]ATP binding [IDA, ISS]cAMP response element binding protein binding [ISS]magnesium ion binding [IDA, ISS]protein binding [IPI]protein kinase binding [IPI]protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

ACTA2