EXT2
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- cellular polysaccharide biosynthetic process [IDA]
- glycosaminoglycan biosynthetic process [IDA, TAS]
- glycosaminoglycan metabolic process [TAS]
- heparan sulfate proteoglycan biosynthetic process [IMP]
- heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process [IMP]
- ossification [IMP]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function- N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity [NAS]
- acetylglucosaminyltransferase activity [IDA]
- glucuronosyltransferase activity [IDA]
- heparan sulfate N-acetylglucosaminyltransferase activity [NAS]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IDA]
- transferase activity, transferring glycosyl groups [IDA]
- N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity [NAS]
- acetylglucosaminyltransferase activity [IDA]
- glucuronosyltransferase activity [IDA]
- heparan sulfate N-acetylglucosaminyltransferase activity [NAS]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IDA]
- transferase activity, transferring glycosyl groups [IDA]
Gene Ontology Cellular Component
EXT1
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- cellular polysaccharide biosynthetic process [IDA]
- glycosaminoglycan biosynthetic process [IDA, TAS]
- glycosaminoglycan metabolic process [TAS]
- heparan sulfate proteoglycan biosynthetic process [IDA, ISS]
- heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process [IMP]
- ossification [IMP]
- signal transduction [TAS]
- skeletal system development [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function- N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity [ISS, NAS]
- acetylglucosaminyltransferase activity [IDA]
- glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity [ISS, NAS]
- glucuronosyltransferase activity [IDA]
- heparan sulfate N-acetylglucosaminyltransferase activity [NAS]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IDA]
- transferase activity, transferring glycosyl groups [IDA]
- N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity [ISS, NAS]
- acetylglucosaminyltransferase activity [IDA]
- glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity [ISS, NAS]
- glucuronosyltransferase activity [IDA]
- heparan sulfate N-acetylglucosaminyltransferase activity [NAS]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IDA]
- transferase activity, transferring glycosyl groups [IDA]
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The BioPlex Network of Human Protein Interactions: Additional Unpublished AP-MS Results (Pre-Publication)
As part of an ongoing effort led by Steve Gygi, Wade Harper, and Ed Huttlin in the Department of Cell Biology at Harvard Medical School, we are systematically profiling the interactions among human proteins using affinity purification mass spectrometry. In this effort, HA-tagged bait proteins obtained from the human ORFeome collection (version 8.1; Marc Vidal) are expressed individually in human ... [more]
Quantitative Score
- 0.999560847 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT (unpublished interaction)
- BioPlex HCT HCT116 cells CompPASS score = 0.99956084727574, threshold = 0.362. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.362 threshold represents the top 2% of scores in HCT116.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| EXT2 EXT1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9957 | BioGRID | 2237336 | |
| EXT2 EXT1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9947 | BioGRID | 3085249 | |
| EXT1 EXT2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID