An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The BioPlex Network of Human Protein Interactions: Additional Unpublished AP-MS Results (Pre-Publication)

Huttlin EL, Pontano-Vaites L, Navarrete-Perea J, Bruckner RJ, Gebreab F, Gygi MP, Thornock A, Fu S, Maenpaa E, Golbazi A, Stricker K, Guha Thakurta S, Zhang T, Rad R, Paulo JA, Harper JW, Gygi SP

As part of an ongoing effort led by Steve Gygi, Wade Harper, and Ed Huttlin in the Department of Cell Biology at Harvard Medical School, we are systematically profiling the interactions among human proteins using affinity purification mass spectrometry. In this effort, HA-tagged bait proteins obtained from the human ORFeome collection (version 8.1; Marc Vidal) are expressed individually in human ... [more]

Status: Pre-Publication Dataset

Quantitative Score

0.827463344 [compPASS Score]

Throughput

High Throughput

Additional Notes

BioPlex HCT (unpublished interaction)
BioPlex HCT HCT116 cells CompPASS score = 0.82746334373043, threshold = 0.362. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.362 threshold represents the top 2% of scores in HCT116.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MVP PARP4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2017)	High	1	BioGRID	2222793
MVP PARP4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	1	BioGRID	3081803
PARP4 MVP	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Kickhoefer VA (1999)	Low	-	BioGRID	-
PARP4 MVP	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Kickhoefer VA (1999)	Low	-	BioGRID	-
PARP4 MVP	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	van Zon A (2002)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

MVP

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein kinase binding [IPI]protein phosphatase binding [IDA]

Gene Ontology Cellular Component

PARP4