BAIT

PSEN1

AD3, FAD, PS-1, PS1, S182
presenilin 1
UBI
GO Process (15)
GO Function (6)
GO Component (27)

External Database Linkouts

BioGRID ORCS | VEGA | HGNC | OMIM | Entrez Gene | RefSeq | UniprotKB | Ensembl | HPRD
Homo sapiens

PREY

PSEN1

AD3, FAD, PS-1, PS1, S182
presenilin 1
UBI
GO Process (15)
GO Function (6)
GO Component (27)

External Database Linkouts

BioGRID ORCS | VEGA | HGNC | OMIM | Entrez Gene | RefSeq | UniprotKB | Ensembl | HPRD
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Dimerization of presenilin-1 in vivo: suggestion of novel regulatory mechanisms leading to higher order complexes.

Hebert SS, Godin C, Tomiyama T, Mori H, Levesque G

A growing body of evidence indicates that presenilins could exist and be active as oligomeric complexes. Using yeast two-hybrid and cell culture analysis, we provide evidence that presenilin-1 (PS1) may self-oligomerize giving rise to specific full-length/full-length homodimers. When expressed in N2A and HEK239T cultured cells, full-length PS1-wt and 5(')myc-PS1-wt form specific homodimers corresponding to twice their molecular weight. The Alzheimer's ... [more]

Biochem. Biophys. Res. Commun. Jan. 31, 2003; 301(1);119-26 [Pubmed: 12535650]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PSEN1 PSEN1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
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Curated By

  • BioGRID