PREY

HSPA4

APG-2, HEL-S-5a, HS24/P52, HSPH2, RY, hsp70, hsp70RY
heat shock 70kDa protein 4
GO Process (3)
GO Function (1)
GO Component (3)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The BioPlex Network of Human Protein Interactions: Additional Unpublished AP-MS Results (Pre-Publication)

Huttlin EL, Pontano-Vaites L, Navarrete-Perea J, Bruckner RJ, Gebreab F, Gygi MP, Thornock A, Fu S, Maenpaa E, Golbazi A, Stricker K, Guha Thakurta S, Zhang T, Rad R, Paulo JA, Harper JW, Gygi SP

As part of an ongoing effort led by Steve Gygi, Wade Harper, and Ed Huttlin in the Department of Cell Biology at Harvard Medical School, we are systematically profiling the interactions among human proteins using affinity purification mass spectrometry. In this effort, HA-tagged bait proteins obtained from the human ORFeome collection (version 8.1; Marc Vidal) are expressed individually in human ... [more]

Status: Pre-Publication Dataset

Quantitative Score

  • 0.601952162 [compPASS Score]

Throughput

  • High Throughput

Additional Notes

  • BioPlex HCT (unpublished interaction)
  • BioPlex HCT HCT116 cells CompPASS score = 0.601952162414431, threshold = 0.362. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.362 threshold represents the top 2% of scores in HCT116.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HSF1 HSPA4
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
HSPA4 HSF1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
HSF1 HSPA4
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
HSF1 HSPA4
Co-localization
Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Low-BioGRID
2469218
HSF1 HSPA4
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
HSF1 HSPA4
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID