Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Cloning and characterization of rat BAT3 cDNA.

Ozaki T, Hanaoka E, Naka M, Nakagawara A, Sakiyama S

HLA-B-associated transcript 3 (BAT3) was originally identified as one of the genes located within human major histocompatibility complex. It encodes a large proline-rich protein with unknown function. In this study, we found that a fragment of the BAT3 gene product interacts with a candidate tumor suppressor, DAN, in the yeast-based two-hybrid system. We cloned the full-length rat BAT3 cDNA from ... [more]

DNA Cell Biol. Jun. 01, 1999; 18(6);503-12 [Pubmed: 10390159]

Throughput

  • Low Throughput

Curated By

  • BioGRID