PREY

SERPINH1

AsTP3, CBP1, CBP2, HSP47, OI10, PPROM, RA-A47, SERPINH2, gp46, PIG14
serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Molecular cloning of a novel ubiquitin-like protein, UBIN, that binds to ER targeting signal sequences.

Matsuda M, Koide T, Yorihuzi T, Hosokawa N, Nagata K

To identify proteins that interact with HSP47, an endoplasmic reticulum (ER)-resident molecular chaperone, a yeast two-hybrid screening was performed using mouse full-length HSP47 including an N-terminal signal sequence as a bait. Analysis of several positive clones led to the identification and cloning of a novel gene, ubin, encoding a ubiquitin-like protein. Unlike other ubiquitin-like proteins, UBIN was shown to interact ... [more]

Biochem. Biophys. Res. Commun. Jan. 19, 2001; 280(2);535-40 [Pubmed: 11162551]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SERPINH1 UBQLN4
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID