DAXX
Gene Ontology Biological Process
- PML body organization [ISO]
- androgen receptor signaling pathway [ISO]
- apoptotic signaling pathway [IGI]
- chromatin remodeling [ISO]
- mitotic cytokinesis [IMP]
- negative regulation of myotube differentiation [ISO]
- negative regulation of nucleic acid-templated transcription [TAS]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [IDA, ISO]
- nucleosome assembly [IMP, ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of apoptotic signaling pathway [IDA, IGI]
- positive regulation of histone phosphorylation [ISO]
- positive regulation of neuron death [ISO]
- positive regulation of protein kinase activity [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of protein ubiquitination [ISO]
- regulation of transcription, DNA-templated [ISO]
Gene Ontology Molecular Function- androgen receptor binding [ISO]
- enzyme binding [ISO]
- histone binding [IDA, ISO]
- p53 binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- protein kinase activator activity [ISO]
- protein kinase binding [ISO]
- transcription coactivator activity [IDA]
- transcription corepressor activity [ISO, TAS]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [ISO]
- androgen receptor binding [ISO]
- enzyme binding [ISO]
- histone binding [IDA, ISO]
- p53 binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- protein kinase activator activity [ISO]
- protein kinase binding [ISO]
- transcription coactivator activity [IDA]
- transcription corepressor activity [ISO, TAS]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
MDM2
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [ISO]
- establishment of protein localization [ISO]
- negative regulation of DNA damage response, signal transduction by p53 class mediator [ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of cell cycle arrest [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- negative regulation of gene expression [ISO]
- negative regulation of protein processing [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- peptidyl-lysine modification [ISO]
- positive regulation of cell cycle [IGI]
- positive regulation of gene expression [ISO]
- positive regulation of mitotic cell cycle [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of protein export from nucleus [ISO]
- protein catabolic process [IDA]
- protein complex assembly [ISO]
- protein destabilization [ISO]
- protein localization to nucleus [ISO]
- protein ubiquitination [IDA, ISO]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISO]
- regulation of protein catabolic process [ISO]
- traversing start control point of mitotic cell cycle [IDA]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The tumour suppressor RASSF1A promotes MDM2 self-ubiquitination by disrupting the MDM2-DAXX-HAUSP complex.
The tumour suppressor p53, which accumulates in response to DNA damage and induces cell-cycle arrest and apoptosis, has a key function in the maintenance of genome integrity. Under normal conditions, the antiproliferative effects of p53 are inhibited by MDM2, a ubiquitin ligase that promotes p53 ubiquitination and degradation. MDM2 is also self-ubiquitinated and degraded. Here, we show that the tumour ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MDM2 DAXX | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| DAXX MDM2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID