An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Insertional mutation of the murine kisimo locus caused a defect in spermatogenesis.

Yanaka N, Kobayashi K, Wakimoto K, Yamada E, Imahie H, Imai Y, Mori C

Spermatogenesis is a developmental process that occurs in several phases and is regulated by a large number of gene products. An insertional transgenic mouse mutant (termed kisimo mouse) has been isolated that results in abnormal germ-cell development, showing abnormal elongated spermatids in the lumina of seminiferous tubules. We cloned the disrupted locus of kisimo and identified a novel testis-specific gene, ... [more]

J. Biol. Chem. May. 19, 2000; 275(20);14791-4 [Pubmed: 10747865]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
THEG CCT5	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Yanaka N (2000)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CCT5

Gene Ontology Biological Process

Gene Ontology Molecular Function

G-protein beta-subunit binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

THEG