Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A family of ADP-ribosylation factor effectors that can alter membrane transport through the trans-Golgi.

Boman AL, Zhang C, Zhu X, Kahn RA

A family of three structurally related proteins were cloned from human cDNA libraries by their ability to interact preferentially with the activated form of human ADP-ribosylation factor 3 (ARF3) in two-hybrid assays. The specific and GTP-dependent binding was later confirmed through direct protein binding of recombinant proteins. The three proteins share large ( approximately 300 residues) domains at their N ... [more]

Mol. Biol. Cell Apr. 01, 2000; 11(4);1241-55 [Pubmed: 10749927]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ARF3 GGA3	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Boman AL (2002)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ARF3

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTPase activity [TAS]

Gene Ontology Cellular Component

GGA3