An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The BioPlex Network of Human Protein Interactions: Additional Unpublished AP-MS Results (Pre-Publication)

Huttlin EL, Pontano-Vaites L, Navarrete-Perea J, Bruckner RJ, Gebreab F, Gygi MP, Thornock A, Fu S, Maenpaa E, Golbazi A, Stricker K, Guha Thakurta S, Zhang T, Rad R, Paulo JA, Harper JW, Gygi SP

As part of an ongoing effort led by Steve Gygi, Wade Harper, and Ed Huttlin in the Department of Cell Biology at Harvard Medical School, we are systematically profiling the interactions among human proteins using affinity purification mass spectrometry. In this effort, HA-tagged bait proteins obtained from the human ORFeome collection (version 8.1; Marc Vidal) are expressed individually in human ... [more]

Status: Pre-Publication Dataset

Quantitative Score

0.88391459 [compPASS Score]

Throughput

High Throughput

Additional Notes

BioPlex HCT (unpublished interaction)
BioPlex HCT HCT116 cells CompPASS score = 0.88391459043128, threshold = 0.362. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.362 threshold represents the top 2% of scores in HCT116.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HAP1 PRKRIR	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9903	BioGRID	3133172

Curated By

BioGRID

Interaction Summary

HAP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

brain-derived neurotrophic factor binding [ISS]ion channel binding [ISS]protein binding [IPI]

Gene Ontology Cellular Component

PRKRIR