Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

MuRF1-dependent regulation of systemic carbohydrate metabolism as revealed from transgenic mouse studies.

Hirner S, Krohne C, Schuster A, Hoffmann S, Witt S, Erber R, Sticht C, Gasch A, Labeit S, Labeit D

Under various pathophysiological muscle-wasting conditions, such as diabetes and starvation, a family of ubiquitin ligases, including muscle-specific RING-finger protein 1 (MuRF1), are induced to target muscle proteins for degradation via ubiquitination. We have generated transgenic mouse lines over-expressing MuRF1 in a skeletal muscle-specific fashion (MuRF1-TG mice) in an attempt to identify the in vivo targets of MuRF1. MuRF1-TG lines were ... [more]

J. Mol. Biol. Jun. 13, 2008; 379(4);666-77 [Pubmed: 18468620]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

TRIM63

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]signal transducer activity [NAS]titin binding [IDA]

Gene Ontology Cellular Component

ENO3