RBPJ
Gene Ontology Biological Process
- DNA recombination [NAS]
- Notch signaling involved in heart development [IC]
- Notch signaling pathway [IMP, TAS]
- angiogenesis [ISS]
- atrioventricular canal development [ISS]
- blood vessel endothelial cell fate specification [ISS]
- blood vessel lumenization [ISS]
- cardiac left ventricle morphogenesis [ISS]
- dorsal aorta morphogenesis [ISS]
- endocardium morphogenesis [ISS]
- epithelial to mesenchymal transition [ISS]
- epithelial to mesenchymal transition involved in endocardial cushion formation [ISS]
- gene expression [TAS]
- labyrinthine layer blood vessel development [ISS]
- negative regulation of ossification [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IMP, ISS]
- negative regulation of transcription, DNA-templated [IDA]
- outflow tract morphogenesis [ISS]
- positive regulation of BMP signaling pathway [ISS]
- positive regulation of ERBB signaling pathway [ISS]
- positive regulation of cardiac muscle cell proliferation [ISS]
- positive regulation of cell proliferation involved in heart morphogenesis [ISS]
- positive regulation of ephrin receptor signaling pathway [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription of Notch receptor target [IDA]
- regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation [ISS]
- transcription initiation from RNA polymerase II promoter [TAS]
- ventricular trabecula myocardium morphogenesis [ISS]
Gene Ontology Molecular Function- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II repressing transcription factor binding [IPI]
- protein binding [IPI]
- recombinase activity [NAS]
- sequence-specific DNA binding transcription factor activity [TAS]
- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II repressing transcription factor binding [IPI]
- protein binding [IPI]
- recombinase activity [NAS]
- sequence-specific DNA binding transcription factor activity [TAS]
Gene Ontology Cellular Component
NOTCH2
Gene Ontology Biological Process
- Notch receptor processing [TAS]
- Notch signaling involved in heart development [IC]
- Notch signaling pathway [TAS]
- apoptotic process [TAS]
- atrial septum morphogenesis [IMP]
- bone remodeling [IMP]
- cell cycle arrest [IDA]
- cell fate determination [TAS]
- cell growth [IDA]
- gene expression [TAS]
- hemopoiesis [TAS]
- intracellular receptor signaling pathway [TAS]
- multicellular organismal development [NAS]
- negative regulation of apoptotic process [TAS]
- negative regulation of cell proliferation [IDA]
- nervous system development [NAS]
- organ morphogenesis [IEP]
- positive regulation of Ras protein signal transduction [IDA]
- pulmonary valve morphogenesis [IMP]
- regulation of transcription, DNA-templated [TAS]
- stem cell maintenance [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The BioPlex Network of Human Protein Interactions: Additional Unpublished AP-MS Results (Pre-Publication)
As part of an ongoing effort led by Steve Gygi, Wade Harper, and Ed Huttlin in the Department of Cell Biology at Harvard Medical School, we are systematically profiling the interactions among human proteins using affinity purification mass spectrometry. In this effort, HA-tagged bait proteins obtained from the human ORFeome collection (version 8.1; Marc Vidal) are expressed individually in human ... [more]
Quantitative Score
- 0.986992368 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT (unpublished interaction)
- BioPlex HCT HCT116 cells CompPASS score = 0.986992368330616, threshold = 0.362. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.362 threshold represents the top 2% of scores in HCT116.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RBPJ NOTCH2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 1445843 | |
| RBPJ NOTCH2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9914 | BioGRID | 1187764 | |
| RBPJ NOTCH2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9557 | BioGRID | 2250771 | |
| RBPJ NOTCH2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9375 | BioGRID | 3138949 | |
| RBPJ NOTCH2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 1506486 | |
| NOTCH2 RBPJ | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID