BAIT

NUMBL

CAG3A, CTG3a, NBL, NUMB-R, NUMBLIKE, NUMBR, TNRC23
numb homolog (Drosophila)-like
Homo sapiens
PREY

TAB2

CHTD2, MAP3K7IP2, TAB-2, RP1-111D6.3
TGF-beta activated kinase 1/MAP3K7 binding protein 2
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

NUMBL interacts with TAB2 and inhibits TNFalpha and IL-1beta-induced NF-kappaB activation.

Ma Q, Zhou L, Shi H, Huo K

The cytokines TNFalpha and IL-1beta induce inflammation through activation of transcription factors NF-kappaB. TAB2 is an adapter protein that facilitates TNFalpha and IL-1beta-mediated NF-kappaB activation. In this work, using yeast two-hybrid system TAB2 was identified to interact with NUMBL. The interaction was further confirmed in vitro and in vivo. PTB domain of NUMBL and C-terminal region are required for their ... [more]

Cell. Signal. Jun. 01, 2008; 20(6);1044-51 [Pubmed: 18299187]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TAB2 NUMBL
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
TAB2 NUMBL
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
NUMBL TAB2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
TAB2 NUMBL
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID