BAIT

BRF1

PCF4, TDS4, transcription factor TFIIIB subunit BRF1, TFIIIB70, L000000193, YGR246C

TFIIIB B-related factor; one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB

GO Process (4)

GO Function (4)

GO Component (1)

Gene Ontology Biological Process

DNA-templated transcriptional open complex formation [IMP]

RNA polymerase III transcriptional preinitiation complex assembly [IDA, IMP]

negative regulation of protein homodimerization activity [IDA]

transposon integration [IDA]

Gene Ontology Molecular Function

DNA binding [IDA]
RNA polymerase III core binding [IDA, IPI]
TFIIIB-type transcription factor activity [IC]
TFIIIC-class transcription factor binding [IDA, IPI]

Gene Ontology Cellular Component

transcription factor TFIIIB complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SMC2

condensin subunit SMC2, L000001927, YFR031C

Subunit of the condensin complex; condensin reorganizes chromosomes during both mitosis and meiosis; essential SMC chromosomal ATPase family member that forms a subcomplex with Smc2p that has ATP-hydrolyzing and DNA-binding activity, but other condensin subunits are required for chromatin binding; required for clustering of tRNA genes at the nucleolus

GO Process (8)

GO Function (6)

GO Component (2)

Gene Ontology Biological Process

meiotic chromosome condensation [IC]

meiotic chromosome separation [IC]

mitotic chromosome condensation [IMP]

mitotic sister chromatid segregation [IMP]

negative regulation of meiotic DNA double-strand break formation [IMP]

rDNA condensation [IMP]

synaptonemal complex assembly [IC]

tRNA gene clustering [IMP]

Gene Ontology Molecular Function

AT DNA binding [IDA]
ATP binding [IDA]
ATPase activity [IDA]
DNA secondary structure binding [IDA]
chromatin binding [IDA]
double-stranded DNA binding [IDA]

Gene Ontology Cellular Component

mitochondrion [IDA]

nuclear condensin complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Clustering of yeast tRNA genes is mediated by specific association of condensin with tRNA gene transcription complexes.

Haeusler RA, Pratt-Hyatt M, Good PD, Gipson TA, Engelke DR

The 274 tRNA genes in Saccharomyces cerevisiae are scattered throughout the linear maps of the 16 chromosomes, but the genes are clustered at the nucleolus when compacted in the nucleus. This clustering is dependent on intact nucleolar organization and contributes to tRNA gene-mediated (tgm) silencing of RNA polymerase II transcription near tRNA genes. After examination of the localization mechanism, we ... [more]

Genes Dev. Aug. 15, 2008; 22(16);2204-14 [Pubmed: 18708579]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

BRF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [IDA]RNA polymerase III core binding [IDA, IPI]TFIIIB-type transcription factor activity [IC]TFIIIC-class transcription factor binding [IDA, IPI]

Gene Ontology Cellular Component

SMC2

Gene Ontology Biological Process

Gene Ontology Molecular Function

AT DNA binding [IDA]ATP binding [IDA]ATPase activity [IDA]DNA secondary structure binding [IDA]chromatin binding [IDA]double-stranded DNA binding [IDA]

Gene Ontology Cellular Component

Affinity Capture-Western

Publication

Clustering of yeast tRNA genes is mediated by specific association of condensin with tRNA gene transcription complexes.

Throughput

Curated By

DNA binding [IDA]
RNA polymerase III core binding [IDA, IPI]
TFIIIB-type transcription factor activity [IC]
TFIIIC-class transcription factor binding [IDA, IPI]

AT DNA binding [IDA]
ATP binding [IDA]
ATPase activity [IDA]
DNA secondary structure binding [IDA]
chromatin binding [IDA]
double-stranded DNA binding [IDA]