PPP1R15A
Gene Ontology Biological Process
- cell cycle arrest [TAS]
- cellular response to DNA damage stimulus [TAS]
- negative regulation of phosphoprotein phosphatase activity [IDA]
- negative regulation of protein dephosphorylation [IDA]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- positive regulation of gene expression [IMP]
- positive regulation of peptidyl-serine dephosphorylation [IDA]
- positive regulation of peptidyl-serine phosphorylation [IMP]
- positive regulation of phosphoprotein phosphatase activity [IDA]
- positive regulation of transcription from RNA polymerase I promoter [IGI]
- positive regulation of translational initiation in response to stress [IC]
- protein localization to endoplasmic reticulum [IMP]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
EIF2S1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
An eIF2?-binding motif in protein phosphatase 1 subunit GADD34 and its viral orthologs is required to promote dephosphorylation of eIF2?.
Transient protein synthesis inhibition, mediated by phosphorylation of the ? subunit of eukaryotic translation initiation factor 2 (eIF2?), is an important protective mechanism cells use during stress conditions. Following relief of the stress, the growth arrest and DNA damage-inducible protein GADD34 associates with the broadly acting serine/threonine protein phosphatase 1 (PP1) to dephosphorylate eIF2?. Whereas the PP1-binding motif on GADD34 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PPP1R15A EIF2S1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID