Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Contribution of the two dsRBM motifs to the double-stranded RNA binding and protein interactions of PACT.

Chukwurah E, Willingham V, Singh M, Castillo-Azofeifa D, Patel RC

PACT is a stress-modulated activator of protein kinase PKR (protein kinase, RNA activated), which is involved in antiviral innate immune responses and stress-induced apoptosis. Stress-induced phosphorylation of PACT is essential for PACT's increased association with PKR leading to PKR activation, phosphorylation of translation initiation factor eIF2?, inhibition of protein synthesis, and apoptosis. PACT-induced PKR activation is negatively regulated by TRBP ... [more]

J Cell Biochem Dec. 01, 2017; 119(4);3598-3607 [Pubmed: 29231267]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PRKRA PRKRA	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Hitti EG (2004)	Low	-	BioGRID	-
PRKRA PRKRA	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Bennett RL (2012)	Low	-	BioGRID	693358
PRKRA PRKRA	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Hitti EG (2004)	Low	-	BioGRID	-
PRKRA PRKRA	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Luck K (2020)	High	-	BioGRID	2742325
PRKRA PRKRA	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Rolland T (2014)	High	-	BioGRID	-
PRKRA PRKRA	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Stelzl U (2005)	High	3	BioGRID	-
PRKRA PRKRA	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Vinayagam A (2011)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

PRKRA

Gene Ontology Biological Process

Gene Ontology Molecular Function

enzyme activator activity [TAS]poly(A) RNA binding [IDA]protein binding [IPI]protein homodimerization activity [IPI]

Gene Ontology Cellular Component