EGLN3
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IEP]
- apoptotic process [IEP]
- cellular response to hypoxia [TAS]
- peptidyl-proline hydroxylation to 4-hydroxy-L-proline [IDA]
- protein hydroxylation [IDA]
- regulation of cell proliferation [IEP]
- regulation of neuron apoptotic process [ISS]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
- response to hypoxia [IEP]
Gene Ontology Molecular Function
USP9X
Gene Ontology Biological Process
- BMP signaling pathway [IDA]
- axon extension [IMP]
- female gamete generation [TAS]
- gene expression [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- neuron migration [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein deubiquitination [IDA]
- regulation of proteasomal protein catabolic process [IBA]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [IMP, TAS]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
USP9X promotes apoptosis in cholangiocarcinoma by modulation expression of KIF1B? via deubiquitinating EGLN3.
Cholangiocarcinoma represents the second most common primary liver malignancy. The incidence rate has constantly increased over the last decades. Cholangiocarcinoma silent nature limits early diagnosis and prevents efficient treatment.Immunoblotting and immunohistochemistry were used to assess the expression profiling of USP9X and EGLN3 in cholangiocarcinoma patients. ShRNA was used to silence gene expression. Cell apoptosis, cell cycle, CCK8, clone formation, shRNA ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| EGLN3 USP9X | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 2463726 | |
| USP9X EGLN3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID