Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Transmembrane protein 168 mutation reduces cardiomyocyte cell surface expression of Nav1.5 through ?B-crystallin intracellular dynamics.

Nguyen LKC, Shimizu A, Soh JEC, Komeno M, Sato A, Ogita H

Transmembrane protein 168 (TMEM168) was found to be localized on the nuclear membrane. A heterozygous mutation (c.1616G>A, p. R539Q) in TMEM168 was identified in patients with Brugada syndrome. This mutation reduced expression of cardiomyocyte sodium channel Nav1.5 via Nedd4-2 E3 ubiquitin ligase-induced ubiquitination and degradation. However, the detailed molecular mechanism provoked by the TMEM168 mutant remains unclear. Here, we demonstrated ... [more]

J Biochem Dec. 28, 2021; 170(5);577-585 [Pubmed: 34086898]

Throughput

  • Low Throughput

Curated By

  • BioGRID