Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

FBXL6 degrades phosphorylated p53 to promote tumor growth.

Li Y, Cui K, Zhang Q, Li X, Lin X, Tang Y, Prochownik EV, Li Y

The ubiquitin-proteasome system regulates many distinct biological processes. Its dysregulation causes various diseases, including but not limited to cancer. In this study, based on the analysis of gene expression in several colorectal cancer (CRC) datasets, we show that FBXL6, a poorly-characterized F-box protein, is amplified, over-expressed, and highly correlated with poor prognosis in human CRC patients. Mechanistically, FBXL6 targets phospho-p53 ... [more]

Cell Death Differ Dec. 01, 2020; 28(7);2112-2125 [Pubmed: 33568778]

Throughput

  • High Throughput

Curated By

  • BioGRID