Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Targeting the p300/NONO axis sensitizes melanoma cells to BRAF inhibitors.

Zhang F, Tang X, Fan S, Liu X, Sun J, Ju C, Liang Y, Liu R, Zhou R, Yu B, Zhang C, Zhang Z, Kang T, Huang G, Lv XB

BRAF inhibitors (BRAFi) that target BRAF V600E kinase, a driver mutation found in 50% of melanomas, show a significant antitumor response, but the common emergence of acquired resistance remains a challenge. Abnormal expression of RAF isoforms CRAF and ARAF reactivates pERK1/2, which plays crucial roles in the acquisition of resistance of melanoma cells. However, the mechanisms of dysregulation of RAF ... [more]

Oncogene Dec. 01, 2020; 40(24);4137-4150 [Pubmed: 34017080]

Throughput

  • High Throughput

Curated By

  • BioGRID