Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

ALS/FTD-causing mutation in cyclin F causes the dysregulation of SFPQ.

Rayner SL, Cheng F, Hogan AL, Grima N, Yang S, Ke YD, Au CG, Morsch M, De Luca A, Davidson JM, Molloy MP, Shi B, Ittner LM, Blair I, Chung RS, Lee A

Previously, we identified missense mutations in CCNF that are causative of familial and sporadic amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Hallmark features of these diseases include the build-up of insoluble protein aggregates as well as the mislocalization of proteins such as transactive response DNA binding protein 43 kDa (TDP-43). In recent years, the dysregulation of SFPQ (splicing factor ... [more]

Hum Mol Genet May. 31, 2021; 30(11);971-984 [Pubmed: 33729478]

Quantitative Score

  • 2.762 [Confidence Score]

Throughput

  • High Throughput

Additional Notes

  • Proximity Label-MS was carried out to identify high-confidence protein interactions with an abundance ratio that was >1.5 fold over control (Fold Abundance ratio over vector control reported)

Curated By

  • BioGRID