SMURF1
Gene Ontology Biological Process
- BMP signaling pathway [IDA, TAS]
- cell differentiation [IDA]
- ectoderm development [TAS]
- negative regulation of BMP signaling pathway [TAS]
- negative regulation of transforming growth factor beta receptor signaling pathway [IDA, TAS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein export from nucleus [IDA]
- protein localization to cell surface [IDA]
- protein polyubiquitination [IDA]
- protein ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
- receptor catabolic process [IDA]
- transforming growth factor beta receptor signaling pathway [TAS]
- ubiquitin-dependent SMAD protein catabolic process [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ARHGEF9
Gene Ontology Biological Process
- apoptotic signaling pathway [TAS]
- ion transmembrane transport [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of apoptotic process [TAS]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
- synaptic transmission [TAS]
- transmembrane transport [TAS]
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Smurf1 directly targets hPEM-2, a GEF for Cdc42, via a novel combination of protein interaction modules in the ubiquitin-proteasome pathway.
Smurf1, a member of HECT-type E3 ubiquitin ligases, regulates cell polarity and protrusive activity by inducing ubiquitination and subsequent proteasomal degradation of the small GTPase RhoA. We report here that hPEM-2, a guanine nucleotide exchange factor for the small GTPase Cdc42, is a novel target of Smurf1. Pulse-chase labeling and a ubiquitination experiment using MG132, a proteasomal inhibitor, indicate that ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ARHGEF9 SMURF1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ARHGEF9 SMURF1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID