An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.

Lv K, Gong C, Antony C, Han X, Ren JG, Donaghy R, Cheng Y, Pellegrino S, Warren AJ, Paralkar VR, Tong W

Impaired ribosome function is the underlying etiology in a group of bone marrow failure syndromes called ribosomopathies. However, how ribosomes are regulated remains poorly understood, as are approaches to restore hematopoietic stem cell (HSC) function loss because of defective ribosome biogenesis. Here we reveal a role of the E3 ubiquitin ligase HectD1 in regulating HSC function via ribosome assembly and ... [more]

Cell Stem Cell Dec. 01, 2020; 28(7);1275-1290.e9 [Pubmed: 33711283]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

HECTD1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]ubiquitin-protein transferase activity [IBA]

Gene Ontology Cellular Component

EXOC1