An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Tumor-associated antigen Prame targets tumor suppressor p14/ARF for degradation as the receptor protein of CRL2Prame complex.

Zhang W, Li L, Cai L, Liang Y, Xu J, Liu Y, Zhou L, Ding C, Zhang Y, Zhao H, Qin J, Shao Z, Wei W, Jia L

Protein Preferentially Expressed Antigen in Melanoma (Prame), a tumor-associated antigen, has been found to frequently overexpress in various cancers, which indicates advanced cancer stages and poor clinical prognosis. Moreover, previous reports noted that Prame functions as a substrate recognizing receptor protein of Cullin RING E3 ligases (CRLs) to mediate potential substrates degradation through Ubiquitin Proteasome System (UPS). However, none of ... [more]

Cell Death Differ Dec. 01, 2020; 28(6);1926-1940 [Pubmed: 33504946]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RBX1 BTBD1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021/pre-pub)	High	0.8016	BioGRID	3288580

Curated By

BioGRID

Interaction Summary

RBX1

Gene Ontology Biological Process

Gene Ontology Molecular Function

NEDD8 transferase activity [IDA]cullin family protein binding [IDA]protein binding [IPI]ubiquitin protein ligase activity [IDA]ubiquitin protein ligase binding [IDA]ubiquitin-protein transferase activity [IDA]ubiquitin-ubiquitin ligase activity [IDA]

Gene Ontology Cellular Component

BTBD1