PREY

HSPD1

CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13
heat shock 60kDa protein 1 (chaperonin)
GO Process (24)
GO Function (11)
GO Component (16)
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.

Zellner S, Schifferer M, Behrends C

Autophagy deficiency in fed conditions leads to the formation of protein inclusions highlighting the contribution of this lysosomal delivery route to cellular proteostasis. Selective autophagy pathways exist that clear accumulated and aggregated ubiquitinated proteins. Receptors for this type of autophagy (aggrephagy) include p62, NBR1, TOLLIP, and OPTN, which possess LC3-interacting regions and ubiquitin-binding domains (UBDs), thus working as a bridge ... [more]

Mol Cell Dec. 18, 2020; 81(6);1337-1354.e8 [Pubmed: 33545068]

Throughput

  • High Throughput

Additional Notes

  • APEX2 proximity label MS carried out to identfy high-confidence protein interactions

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
OPTN HSPD1
Co-localization
Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Low-BioGRID
-

Curated By

  • BioGRID