Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.

Zellner S, Schifferer M, Behrends C

Autophagy deficiency in fed conditions leads to the formation of protein inclusions highlighting the contribution of this lysosomal delivery route to cellular proteostasis. Selective autophagy pathways exist that clear accumulated and aggregated ubiquitinated proteins. Receptors for this type of autophagy (aggrephagy) include p62, NBR1, TOLLIP, and OPTN, which possess LC3-interacting regions and ubiquitin-binding domains (UBDs), thus working as a bridge ... [more]

Mol Cell Dec. 18, 2020; 81(6);1337-1354.e8 [Pubmed: 33545068]

Throughput

  • High Throughput

Additional Notes

  • APEX2 proximity label MS carried out to identfy high-confidence protein interactions

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TAX1BP1 RAB5C
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-

Curated By

  • BioGRID