BAIT
PKD2
C030034P18Rik, PC2, TRPP2
polycystic kidney disease 2
GO Process (37)
GO Function (21)
GO Component (21)
Gene Ontology Biological Process
- JAK-STAT cascade [IDA]
- calcium ion transmembrane transport [IDA, ISO]
- calcium ion transport [IMP, ISO]
- cell cycle arrest [IDA]
- cellular calcium ion homeostasis [IMP]
- cellular response to fluid shear stress [IMP, ISO]
- cellular response to hydrostatic pressure [ISO]
- cellular response to osmotic stress [ISO]
- cytoplasmic sequestering of transcription factor [ISO]
- detection of mechanical stimulus [IMP]
- detection of nodal flow [IMP]
- determination of left/right symmetry [IMP]
- determination of liver left/right asymmetry [ISO]
- embryonic placenta development [IMP]
- heart development [IMP]
- heart looping [ISO]
- kidney development [IGI, IMP]
- liver development [IGI]
- negative regulation of G1/S transition of mitotic cell cycle [ISO]
- negative regulation of ryanodine-sensitive calcium-release channel activity [IDA]
- placenta blood vessel development [IMP]
- positive regulation of cell cycle arrest [ISO]
- positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle [ISO]
- positive regulation of cytosolic calcium ion concentration [ISO]
- positive regulation of gene expression [IMP]
- positive regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity [ISO]
- positive regulation of nitric oxide biosynthetic process [IMP, ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- potassium ion transmembrane transport [IDA]
- regulation of cAMP metabolic process [IMP]
- regulation of calcium ion import [ISO]
- regulation of cell proliferation [ISO]
- release of sequestered calcium ion into cytosol [IMP, ISO]
- renal system development [IGI]
- renal tubule morphogenesis [IMP]
- sodium ion transmembrane transport [ISO]
- transmembrane transport [IMP]
Gene Ontology Molecular Function- ATPase binding [IPI]
- HLH domain binding [ISO]
- actinin binding [ISO]
- alpha-actinin binding [ISO]
- calcium channel activity [IDA, TAS]
- calcium ion binding [IDA]
- calcium-induced calcium release activity [ISO]
- channel activity [IMP]
- cytoskeletal protein binding [ISO]
- identical protein binding [IPI, ISO]
- ion channel binding [ISO]
- muscle alpha-actinin binding [ISO]
- phosphoprotein binding [ISO]
- potassium channel activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- receptor binding [IPI, ISO]
- voltage-gated calcium channel activity [ISO]
- voltage-gated cation channel activity [ISO]
- voltage-gated ion channel activity [ISO]
- voltage-gated sodium channel activity [ISO]
- ATPase binding [IPI]
- HLH domain binding [ISO]
- actinin binding [ISO]
- alpha-actinin binding [ISO]
- calcium channel activity [IDA, TAS]
- calcium ion binding [IDA]
- calcium-induced calcium release activity [ISO]
- channel activity [IMP]
- cytoskeletal protein binding [ISO]
- identical protein binding [IPI, ISO]
- ion channel binding [ISO]
- muscle alpha-actinin binding [ISO]
- phosphoprotein binding [ISO]
- potassium channel activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- receptor binding [IPI, ISO]
- voltage-gated calcium channel activity [ISO]
- voltage-gated cation channel activity [ISO]
- voltage-gated ion channel activity [ISO]
- voltage-gated sodium channel activity [ISO]
Gene Ontology Cellular Component
- basal cortex [IDA, ISO]
- basal plasma membrane [ISO]
- cell-cell junction [IDA]
- ciliary basal body [IDA, ISO]
- cilium [IDA, IMP]
- cytoplasm [IDA, ISO]
- cytosol [IMP]
- endoplasmic reticulum [IDA, IMP, ISO, TAS]
- endoplasmic reticulum membrane [ISO]
- extracellular vesicular exosome [ISO]
- filamentous actin [ISO]
- integral component of cytoplasmic side of endoplasmic reticulum membrane [ISO]
- integral component of lumenal side of endoplasmic reticulum membrane [ISO]
- integral component of plasma membrane [ISO]
- lamellipodium [ISO]
- membrane [ISO]
- mitotic spindle [IDA, ISO]
- motile primary cilium [IDA]
- nonmotile primary cilium [IDA]
- plasma membrane [IDA, ISO]
- polycystin complex [IDA]
Mus musculus
PREY
VCP
3110001E05, CDC48, p97, p97/VCP, RP23-124L1.5
valosin containing protein
GO Process (19)
GO Function (13)
GO Component (15)
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- ER to Golgi vesicle-mediated transport [ISO]
- ER-associated ubiquitin-dependent protein catabolic process [ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- aggresome assembly [IGI]
- cellular response to DNA damage stimulus [ISO]
- double-strand break repair [ISO]
- positive regulation of Lys63-specific deubiquitinase activity [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of protein K63-linked deubiquitination [ISO]
- positive regulation of protein catabolic process [ISO]
- positive regulation of protein complex assembly [ISO]
- protein N-linked glycosylation via asparagine [ISO]
- protein hexamerization [ISO]
- protein homooligomerization [ISO]
- protein ubiquitination [ISO]
- retrograde protein transport, ER to cytosol [ISO]
- translesion synthesis [ISO]
- ubiquitin-dependent protein catabolic process [IGI]
Gene Ontology Molecular Function- ADP binding [ISO]
- ATP binding [ISO]
- ATPase activity [ISO]
- deubiquitinase activator activity [ISO]
- identical protein binding [ISO]
- poly(A) RNA binding [ISO]
- polyubiquitin binding [IDA, ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein domain specific binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [ISO]
- ubiquitin-specific protease binding [ISO]
- ADP binding [ISO]
- ATP binding [ISO]
- ATPase activity [ISO]
- deubiquitinase activator activity [ISO]
- identical protein binding [ISO]
- poly(A) RNA binding [ISO]
- polyubiquitin binding [IDA, ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein domain specific binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [ISO]
- ubiquitin-specific protease binding [ISO]
Gene Ontology Cellular Component
- Hrd1p ubiquitin ligase complex [ISO]
- cytoplasm [ISO]
- cytosol [ISO]
- endoplasmic reticulum [ISO]
- endoplasmic reticulum membrane [ISO]
- extracellular vesicular exosome [ISO]
- intracellular membrane-bounded organelle [ISO]
- lipid particle [ISO]
- myelin sheath [IDA]
- nucleoplasm [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- proteasome complex [ISO]
- protein complex [IPI]
- site of double-strand break [ISO]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Polycystin-2 is regulated by endoplasmic reticulum-associated degradation.
Endoplasmic reticulum(ER)-associated degradation (ERAD) is an essential process for cell homeostasis and remains not well understood. During ERAD, misfolded proteins are recognized, ubiquitinated on ER and subsequently retro-translocated/dislocated from ER to the 26S proteasome in the cytosol for proteolytic elimination. Polycystin-2 (PC2), a member of the transient receptor potential superfamily of cation channels, is a Ca channel mainly located on ... [more]
Hum. Mol. Genet. Apr. 15, 2008; 17(8);1109-19 [Pubmed: 18178578]
Throughput
- Low Throughput
Curated By
- BioGRID