PREY

DLGAP2

DAP-2, SAPAP2
discs, large (Drosophila) homolog-associated protein 2
GO Process (2)
GO Function (3)
GO Component (3)
Rattus norvegicus

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95.

Kornau HC, Schenker LT, Kennedy MB, Seeburg PH

The N-methyl-D-aspartate (NMDA) receptor subserves synaptic glutamate-induced transmission and plasticity in central neurons. The yeast two-hybrid system was used to show that the cytoplasmic tails of NMDA receptor subunits interact with a prominent postsynaptic density protein PSD-95. The second PDZ domain in PSD-95 binds to the seven-amino acid, COOH-terminal domain containing the terminal tSXV motif (where S is serine, X ... [more]

Science Sep. 22, 1995; 269(5231);1737-40 [Pubmed: 7569905]

Throughput

  • Low Throughput

Curated By

  • BioGRID