BAIT

MOT1

BUR3, END10, LPF4, BTAF1, L000001136, L000004559, S000029158, YPL082C

Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity; may have a role in ensuring that soluble TBP is available to bind TATA-less promoters; forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA

GO Process (5)

GO Function (3)

GO Component (3)

Gene Ontology Biological Process

negative regulation of transcription, DNA-templated [IMP]

rRNA processing [IMP]

regulation of RNA polymerase II transcriptional preinitiation complex assembly [IMP]

regulation of transcription from RNA polymerase II promoter [IDA]

transcription of nuclear large rRNA transcript from RNA polymerase I promoter [IDA, IMP]

Gene Ontology Molecular Function

ATPase activity [IDA]
DNA binding [IDA]
TBP-class protein binding [IPI]

Gene Ontology Cellular Component

mitochondrion [IDA]

nuclear chromosome [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

TAF12

TAF61, TAF68, TafII61, TafII68, L000003462, YDR145W

Subunit (61/68 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A

GO Process (4)

GO Function (5)

GO Component (3)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IMP]

chromatin modification [IDA, IMP]

histone acetylation [IDA]

transcription from RNA polymerase II promoter [IDA, IMP]

Gene Ontology Molecular Function

RNA polymerase II activating transcription factor binding [IDA, IPI]
RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]
TBP-class protein binding [IDA]
chromatin binding [IDA]
protein complex scaffold [IMP]

Gene Ontology Cellular Component

SAGA complex [IDA]

SLIK (SAGA-like) complex [IDA]

transcription factor TFIID complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A proteomics analysis of yeast Mot1p protein-protein associations: insights into mechanism.

Arnett DR, Jennings JL, Tabb DL, Link AJ, Weil PA

Yeast Mot1p, a member of the Snf2 ATPase family of proteins, is a transcriptional regulator that has the unusual ability to both repress and activate mRNA gene transcription. To identify interactions with other proteins that may assist Mot1p in its regulatory processes, Mot1p was purified from replicate yeast cell extracts, and Mot1p-associated proteins were identified by coupled multidimensional liquid chromatography ... [more]

Mol. Cell Proteomics Nov. 01, 2008; 7(11);2090-106 [Pubmed: 18596064]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MOT1 TAF12	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Arnett DR (2008)	Low	-	BioGRID	-
TAF12 MOT1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
MOT1 TAF12	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	2	BioGRID	3602479
TAF12 MOT1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.1751	BioGRID	1925644

Curated By

BioGRID

Interaction Summary

MOT1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity [IDA]DNA binding [IDA]TBP-class protein binding [IPI]

Gene Ontology Cellular Component

TAF12

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II activating transcription factor binding [IDA, IPI]RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]TBP-class protein binding [IDA]chromatin binding [IDA]protein complex scaffold [IMP]

Gene Ontology Cellular Component

Affinity Capture-Western

Publication

A proteomics analysis of yeast Mot1p protein-protein associations: insights into mechanism.

Throughput

Related interactions

Curated By

ATPase activity [IDA]
DNA binding [IDA]
TBP-class protein binding [IPI]

RNA polymerase II activating transcription factor binding [IDA, IPI]
RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]
TBP-class protein binding [IDA]
chromatin binding [IDA]
protein complex scaffold [IMP]