Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.

Zellner S, Schifferer M, Behrends C

Autophagy deficiency in fed conditions leads to the formation of protein inclusions highlighting the contribution of this lysosomal delivery route to cellular proteostasis. Selective autophagy pathways exist that clear accumulated and aggregated ubiquitinated proteins. Receptors for this type of autophagy (aggrephagy) include p62, NBR1, TOLLIP, and OPTN, which possess LC3-interacting regions and ubiquitin-binding domains (UBDs), thus working as a bridge ... [more]

Mol Cell Dec. 18, 2020; 81(6);1337-1354.e8 [Pubmed: 33545068]

Throughput

  • High Throughput

Additional Notes

  • Affinity capture MS carried out to identify high-confidence protein interactions

Curated By

  • BioGRID