An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Tandem affinity purification of AtTERT reveals putative interaction partners of plant telomerase in vivo.

Majerska J, Schrumpfova PP, Dokladal L, Schorova S, Stejskal K, Oboril M, Honys D, Kozakova L, Polanska PS, Sykorova E

The life cycle of telomerase involves dynamic and complex interactions between proteins within multiple macromolecular networks. Elucidation of these associations is a key to understanding the regulation of telomerase under diverse physiological and pathological conditions from telomerase biogenesis, through telomere recruitment and elongation, to its non-canonical activities outside of telomeres. We used tandem affinity purification coupled to mass spectrometry to ... [more]

Protoplasma Jul. 01, 2017; 254(4);1547-1562 [Pubmed: 27853871]

Throughput

High Throughput

Additional Notes

Stable transformed cell suspension culture, tandem affinity purification coupled to mass spectrometry, TAP-tagged interactor A (bait) capture of Interactor B (prey) identified by MS

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TERT AT5G35680	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Fulneckova J (2021)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

TERT

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]telomerase activity [IDA]telomeric template RNA reverse transcriptase activity [ISS]

Gene Ontology Cellular Component

AT5G35680