BAIT

CBL

Cbl proto-oncogene, E3 ubiquitin protein ligase
GO Process (0)
GO Function (0)
GO Component (0)
Chlorocebus sabaeus
PREY

KIT

C-Kit, CD117, PBT, SCFR
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
GO Process (53)
GO Function (5)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Direct binding of Cbl to Tyr568 and Tyr936 of the stem cell factor receptor/c-Kit is required for ligand-induced ubiquitination, internalization and degradation.

Masson K, Heiss E, Band H, Roennstrand L

The ubiquitin E3 ligase Cbl has been shown to negatively regulate tyrosine kinase receptors, including the stem cell factor receptor/c-Kit. Impaired recruitment of Cbl to c-Kit results in a deregulated positive signalling that eventually can contribute to carcinogenesis. Here, we present results showing that Cbl is activated by the SFKs (Src family kinases) and recruited to c-Kit in order to ... [more]

Biochem. J. Oct. 01, 2006; 399(1);59-67 [Pubmed: 16780420]

Throughput

  • Low Throughput

Curated By

  • BioGRID