BEM1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SPA2
Gene Ontology Biological Process
- bipolar cellular bud site selection [IMP]
- budding cell apical bud growth [IGI, IMP]
- establishment of cell polarity [IMP]
- invasive filamentous growth [IGI]
- mating projection assembly [IGI]
- positive regulation of actin cytoskeleton reorganization [IGI, IMP]
- pseudohyphal growth [IMP]
- regulation of initiation of mating projection growth [IMP]
- regulation of protein localization [IMP]
- regulation of termination of mating projection growth [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
A time-resolved interaction analysis of Bem1 reconstructs the flow of Cdc42 during polar growth.
Cdc42 organizes cellular polarity and directs the formation of cellular structures in many organisms. By locating Cdc24, the source of active Cdc42, to the growing front of the yeast cell, the scaffold protein Bem1, is instrumental in shaping the cellular gradient of Cdc42. This gradient instructs bud formation, bud growth, or cytokinesis through the actions of a diverse set of ... [more]
Throughput
- Low Throughput
Additional Notes
- split-ubiquitin
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SPA2 BEM1 | Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene. | Low | - | BioGRID | 429729 | |
| BEM1 SPA2 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.5076 | BioGRID | 359193 | |
| BEM1 SPA2 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.445 | BioGRID | 2083000 |
Curated By
- BioGRID