A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Telomerase Interaction Partners-Insight from Plants.

Fulneckova J, Dokladal L, Kolarova K, Nespor Dadejova M, Prochazkova K, Gomelska S, Sivcak M, Adamusova K, Lycka M, Peska V, Dvorackova M, Sykorova E

Telomerase, an essential enzyme that maintains chromosome ends, is important for genome integrity and organism development. Various hypotheses have been proposed in human, ciliate and yeast systems to explain the coordination of telomerase holoenzyme assembly and the timing of telomerase performance at telomeres during DNA replication or repair. However, a general model is still unclear, especially pathways connecting telomerase with ... [more]

Int J Mol Sci Dec. 29, 2021; 23(1); [Pubmed: 35008793]

Throughput

Low Throughput

Additional Notes

Bimolecular Fluorescence Complementation (BiFC)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TERT AT5G27120	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Majerska J (2017)	High	-	BioGRID	3308104

Curated By

BioGRID

Interaction Summary

TERT

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]telomerase activity [IDA]telomeric template RNA reverse transcriptase activity [ISS]

Gene Ontology Cellular Component

AT5G27120