BAIT

ANXA2

ANX2, ANX2L4, CAL1H, HEL-S-270, LIP2, LPC2, LPC2D, P36, PAP-IV
annexin A2
Homo sapiens
PREY

TFEB

ALPHATFEB, BHLHE35, TCFEB, RP4-696P19.3
transcription factor EB
Alzheimer's Disease Project
Autophagy Project
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

TRIM21-regulated Annexin A2 plasma membrane trafficking facilitates osteosarcoma cell differentiation through the TFEB-mediated autophagy.

Zhang HT, Zeng Q, Wu B, Lu J, Tong KL, Lin J, Liu QY, Xu L, Yang J, Liu X, Liu W, Zhang YF, Lian Q, Liu L, Gao X

Osteosarcoma (OS) is the most common primary malignant bone tumor in children and adolescents, which is characterized by dysfunctional autophagy and poor differentiation. Our recent studies have suggested that the tripartite motif containing-21 (TRIM21) plays a crucial role in regulating OS cell senescence and proliferation via interactions with several proteins. Yet, its implication in autophagy and differentiation in OS is ... [more]

Cell Death Dis Dec. 06, 2020; 12(1);21 [Pubmed: 33414451]

Throughput

  • Low Throughput

Curated By

  • BioGRID