BAIT

PRKRA

DYT16, PACT, RAX, HSD14
protein kinase, interferon-inducible double stranded RNA dependent activator
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The PKR activator, PACT, becomes a PKR inhibitor during HIV-1 replication.

Clerzius G, Shaw E, Daher A, Burugu S, Gelinas JF, Ear T, Sinck L, Routy JP, Mouland AJ, Patel RC, Gatignol A

HIV-1 translation is modulated by the activation of the interferon (IFN)-inducible Protein Kinase RNA-activated (PKR). PKR phosphorylates its downstream targets, including the alpha subunit of the eukaryotic translation Initiation Factor 2 (eIF2?), which decreases viral replication. The PKR Activator (PACT) is known to activate PKR after a cellular stress. In lymphocytic cell lines, HIV-1 activates PKR only transiently and not ... [more]

Retrovirology Sep. 11, 2013; 10();96 [Pubmed: 24020926]

Throughput

  • Low Throughput

Curated By

  • BioGRID